- mRNA is the most abundant type in living cells (by percent)?（ ）
- RNA Pol II is the enzyme synthesizing RNA in mammals. It is not susceptible to amanitin. （ ）
- DNA sequences and DNA-binding proteins control transcription. The DNA sequences are named as cis-acting elements, such as the TATA box, enhancers and other elements.（ ）
- Mismatch repair system can increase the DNA replication fidelity by 100-1000 times.（ ）
- Both genomic DNA and cDNA can be used as target DNA in molecular cloning.（ ）
- Both prokaryotic and eukaryotic DNA polymerases are composed of one polypeptide chain.（ ）
- Gene chip can also be called DNA microarray.（ ）
- In the eukaryotic trans-acting factors, activator proteins usually bind to the promoters directly.（ ）
- Stop codons are never translated. （ ）
- The protein product coded by myc gene is transcription factor.（ ）
- Plasmids can accommodate up to 45kb foreign DNA.（ ）
- Ribosomes become attached to transfer RNA (tRNA) molecules before the translation process can begin in Prokaryotes.（ ）
- Only the environmental agents can induce the occurrence of DNA damages.（ ）
- Trans-acting transcription factors are the DNA-binding proteins that bind with high specificity to DNA regulatory sequences and facilitate the binding and positioning of RNA Pol for the synthesis of pre-RNA.（ ）
- Both prokaryotic and eukaryotic transcriptions are accompanied by translation. （ ）
- The base in DNA _____
- tRNA in prokaryotic cell contains （ ）
- tRNA in eukaryotic cell contains _____
- EF-Tu and EF-Ts are involved in protein biosynthesis:（ ）
- What are the lipoproteins obtained in agarose gel electrophoresis of blood lipoprotein experiment?（ ）
- Reverse transcriptase can（ ）
- The advantages of prokaryotic expression system include（ ）
- Which of the following steps could be regulated to influence the amount of the product of a gene in eukaryotes?（ ）
- Protein synthesis in prokaryotic cells is characterized by:（ ）
- Which of the following are true about the enhancers?（ ）
- The final products of gene expression include:（ ）
- In DNA, phosphodiester bonds form with hydroxyl groups of carbons number ________ and________ of each deoxyribose sugar.（ ）
- Which of the classes of RNA molecules is/are part of the process oftranslation?（ ）
- The base in DNA（ ）
- The Rho protein is involved in the _____ stage of transcription. （ ）
- A “mini-gene” has the base sequence TACCCGTGCACG. If the T at the beginning of the sequence is deleted, what will be the consequence?（ ）
- .What are the steps of PCR?（ ）
- Which of the following occurs first in prokayotic transcription?（ ）
- Of all the possible amino acids, all living organisms make use of only the same 20 amino acids. This supports the idea that:（ ）
- Among the statements about 3’ poly(A) tail which are correct?（ ）
- DNA molecules Used as probes must:（ ）
- In insertional inactivation selection, Which of the following bacteria can grow in the presence of tetracycline（ ）
- The tool enzyme used for combination of foreign DNA and vector is（ ）
- What is the molecule size of the target band of PCR?（ ）
- Amount of T in RNA is 0.25mol，so amount of A is_____.
- The secondary structure of DNA is _____
- Which can recognize the N-terminus of the nascent peptide chain of the secreted protein?（ ）
- Which of the following is the most essential for a gene expression?（ ）
- What is the extension temperature of the PCR?（ ）
- tRNA is used as _____ in translation.（ ）
- The Shine-Dalgarno sequence is found on the（ ）.
- Which enzyme below can not form 3’,5’- phosphodiester bond?（ ）
- Which is wrong below as described for directly undoing DNA damages repair?（ ）
- In α-complementation selection, the foreign DNA will be inserted into（ ）
- Which base substitution below belongs to “Transition” point mutation?（ ）
- which bond an amino acid is linked to the tRNA?（ ）
- The technology of isotope-labeled probes to detect the RNA molecule on the NC membrane is called:（ ）
- mRNA is used as _____ in translation.
- Please arrange the following proteins by the order of participating in DNA replication（ ）① Primase② Helicase③ Single-strand binding proteins④ DNA polymerase I
- Which band runs fastest in the agarose gel electrophoresis of blood lipoprotein experiment?（ ）
- Which regulator could inhibits gene transcription？（ ）
- Which of the following is expressed at relatively constant rate in almost all the cells?（ ）
- Which is the most commonly used elements of eukaryotic promoter?（ ）
- Which transcription factor can recognize and bind to TATA box?（ ）
- Which one of the following is tumor suppressor gene?（ ）
- According to Chargaffʹs observations of nucleotide composition of DNA samples（ ）
- Which is FALSE description of DNA replication（ ）
- Which is not true of the different conformations of DNA?（ ）
- What would be the tRNA anticodon for the mRNA codon CAG?（ ）
- In the peptide chain synthesis, which site aminoacyl-tRNA bound with the prokaryotic ribosome?（ ）
- Which of the following occurs as a result of translocation?（ ）
- In the regulation of trp operon, when the tryptophan is scarce in medium, which statement is true?（ ）
- Which protein factor has translocase activity in prokaryotes?（ ）
- Which statement is true?（ ）
- In which process GG-NER and TC-NER show different （ ）
- Starting with 15N15N (heavy)DNA, and after 3 generations in the 14N medium, E. coli cells will produce the following band(s) in density-gradient centrifugation as below:（ ）
- In lac operon, what is the right order of the three regulatory sequences?（ ）
- The corresponding codon to anticodon UCC is（ ）
- Among the following factors, the one that does not participate in the translation process of prokaryotes is（ ）
- During protein synthesis, the activation site of amino acids is（ ）
- Which of the following sequences is palindrome sequence（ ）
- Amount of G in RNA is 0.25mol，so amount of C is（ ）.
- Which of the following is an advantage of phage vectors over plasmid vectors?（ ）
- Which enzyme elongates the leading strand in E. coli?（ ）
- All the RNAs below are transcribed by RNA pol I except （ ）
- Mitochondrial DNA is replicated by（ ）
- Which RNA polymerase transcribes the gene of 28S rRNA?（ ）
- In most melanoma, oncogenic BRAF was activated by（ ）
- What the signal peptide acts?（ ）
- Which of the following eukaryotic ligases involves in filling the gap between the Okazaki fragments（ ）
- What is the order of lipoprotein from the cathode to anode?（ ）
- What are the solutions used in the experiment?（ ）
- What is the bacterial used in the experiment?（ ）
- What are the steps of PCR?（ ）
- What is the annealing temperature of the PCR?（ ）
- What is the staining reagent in the experiment?（ ）
- In blue/white screening, the bacteria containing wild vectors will yield white colonies.（ ）
- Restriction enzymes randomly cleave DNA（ ）
- Plasmids are usually introduced into bacterial cells by.（ ）
- Based on the naming rules, the first letter of a restriction endonuclease means（ ）
- Isocaudarner presents（ ）
- Which is not necessary as part of prokaryotic expression vector?（ ）
- In insertional inactivation screening, the bacteria that are sensitive to tetracycline and resistant to ampicillin should be transformed with（ ）
- T4 DNA ligase requires（ ）
- Which of the following DNA molecules are commonly used in molecular cloning（ ）
- Which of the following methods is used for isolation of foreign DNA（ ）
- The loading capacity of plasmids is usually （ ）
- Which of the following statements is not true concerning Type II restriction endonucleases?（ ）
- Which technology can be used to identify DNA?（ ）
- In the PCR reaction, the purpose of annealing is to make the combination of primer and template. （ ）
- The basic components of the PCR system does not include:（ ）
- Which are right about molecular hybridization?（ ）
- Protein chip technology has the characteristics of fast and high throughput, it can be used to analyze thousands of proteins in the whole genome level at the same time.（ ）
- The basic steps of PCR reaction are: （ ）
- The mechanisms of tumor suppressor gene inactivation include（ ）
- In most chronic myelogenous leukemia, tumorigenesis is caused by oncogene amplification（ ）
- The following changes promote oncogene activation, except （ ）
- Which statement is true about oncogenes?（ ）
- Which one of the following is oncogene?（ ）
- The active chromatin usually shows low level of methylation.（ ）
- Enhancers must locate on the upstream region of promoter.（ ）
- If a gene is expressed only in certain organs or tissues, this is called （ ）
- In the regulation of lac operon, when the medium contains both glucose and lactose, which statement is true?（ ）
- If a gene is expressed at relatively constant rate in almost all the cells, it is called a(an) （ ）
- Which one is NOT a cis-acting element?（ ）
- In lac operon, repressor protein could bind to( )sequence.（ ）
- The most efficient step of gene expression regulation is （ ）
- Which is NOT a final product of gene expression?（ ）
- In the regulation of lac operon, when the medium contains only glucose, which statement is true?（ ）
- The prokaryotic operator sequence is usually （ ） .
- Which is NOT the basal cis-acting elements in eukaryotes?（ ）
- The genetic code has four letters, A, T, G, and C. （ ）
- Polypeptides unfold in the cytoplasm of a cell to form a functional protein.（ ）
- Messenger RNA (mRNA) is made by a process called translation.（ ）
- The specific sequence on the N terminus of secretory protein is called （ ）
- We usually call codons contains 5 characteristics, among which the degeneracy of the genetic code means?（ ）
- Which protein factor has the translocase enzyme activity in eukaryotes:（ ）
- Among the following factors, which one could deliver the aminoacyl-tRNA to the A site of ribosome?（ ）
- The Shine-Dalgarno sequence is found on the ________. （ ）
- The energy required for the step of peptide chain elongation in prokaryotic is? （ ）
- A gene has the base sequence ACCCGTGCACGT. If the A at the beginning of the sequence is deleted, what will be happen?（ ）
- Which bond is an amino acid linked to the tRNA?（ ）
- Eukaryotic transcriptions is accompanied by translation.（ ）
- RNA Pol II is the enzyme synthesizing hnRNA in eukaryotes. It is not susceptible to amanitin.（ ）
- Which RNA polymerase transcribes the gene of insulin? （ ）
- The functions of 5’ Cap of eukaryotic mRNA include（ ）
- Cis-acting elements and trans-acting factors control transcription. The cis-acting elements include the TATA box, enhancers and other elements et al.（ ）
- In prokaryotes, RNA polymerase can bind upstream sequences of promoters directly. Eukaryotes need some proteins to facilitate the binding. What are the names of these proteins? （ ）
- A mutation to this sequence in eukaryotic mRNA will affect the process by which the 3'-end poly-A tail is added to the mRNA.（ ）
- Which of the following steps occurs first in prokayotic transcription?（ ）
- Which transcription factor can recognize and bind to TATA box? （ ）
- Which component of the prokaryote RNA polymerase holoenzyme is involved in recognizing promoters specific to certain types of genes? （ ）
- Which type of DNA point mutations will not lead to the change in amino acid （ ）.
- During mismatch repair in prokaryotes, the newly synthesized DNA is recognized by the lack of ( ) modification（ ）
- which base substitution below belongs to “Transition” point mutation.（ ）
- DNA damage is the main cause for the increased frequency of gene mutation. （ ）
- In which process GG-NER and TC-NER show different（ ）
- Base excision repair is one of DNA repair pathways to corrects large base lesions .（ ）
- The deficiency of NER repair proteins is susceptible to_ disease.（ ）
- Which is wrong below as described for directly undoing DNA damages repair.（ ）
- Which of the following DNA damages belongs to exogenous DNA damage （ ）.
- During double stranded DNA break repair, NHEJ shows more precise compared to HR. （ ）
- The Okazaki fragment of eukaryotes is longer than that of prokaryotes.（ ）
- Reverse transcriptase exerts the functions of RNA dependent DNA polymerase and DNA dependent DNA polymerase.（ ）
- Which mechanism can explain two strands are synthesized in the opposite direction, in the replication fork?（ ）
- RNA primer in DNA replication （ ）
- What are the differences of eukaryotic DNA replication from prokaryotes? （ ）
- Which is FALSE about the termination of prokaryotic DNA replication?（ ）
- Although mammalian genome is huge, cells can replicate the genome in just a few minutes.Which is the most possible reason?（ ）
- Mitochondrial DNA replicates in（ ）
- DNA replication needs（ ）
- Which is FALSE about DNA pol III in prokaryotes?（ ）
- Based on Meselson-Stahl experiment, each daughter DNA molecule is composed of（ ）
- Which is TURE about the initiation of prokaryotic DNA replication?（ ）
- Sequences that are removed from the primary RNA transcript in eukaryotes are called________.（ ）
- All housekeeping genes have weak promotors and are regulated to maintain their geneproducts at constant low level concentrations.（ ）
- The regulators of eukaryotic gene expression consist of________.（ ）
- Which is not a feature of enhancer？（ ）
- Which character is not true for eukaryotic genes?（ ）
- All genomes are composed of RNA.（ ）
- Single strand DNA 5’-CGGTA-3’ can anneal with the follow RNA _____（ ）
- The corresponding anticodon to codon ACG is___ （ ）
- The common component composed of DNA and RNA is _____（ ）
- mRNA is used as _____ in translation.（ ）
- The Nucleic acid will get maximum UV absorbance at wavelength_____（ ）
- Amount of T in RNA is 0.25mol，so amount of A is_____. （ ）
- Stem-loop is a type of secondary structure not usually found in tRNA.（ ）
- tRNA in eukaryotic cell contains _____（ ）
- The base in DNA _____（ ）
- The secondary structure of DNA is _____（ ）
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