第十一章单元测试
5’-End labeling is performed using （ ）to add a radioactive phosphate to nucleic acids with a free 5’-hydroxyl group, which can be created by dephosphorylation using alkaline phosphatase.
In Southern blotting, the washing step should be carried out at （ ）℃ below Tm of the probe and target sequences.
Sanger chain-termination method of DNA sequencing uses （ ） to terminate enzymically synthesized copies of a template.
The sequence of the PCR steps is（ ）。
Gel retardation shows the actual region of sequence with which the protein interacts。（ ）
A:polynucleotide kinase B:T4 DNA ligase C:restriction enzymes D:DNA polymerase
答案:polynucleotide kinase
A:12 B:5 C:6 D:10
A:dNTPs B:None of the above C:ddNTPs D:NTPs
A:Denaturation→Annealing→Extension B:Denaturation→Extension→Annealing C:Extension→Annealing→Denaturation D:Annealing→Denaturation→Extension
A:错 B:对
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