第十六章单元测试
- The optimal cell system to express human protein is（ ）
- A certain restricion endonuclease cut DNA by means of GGG▼CGCCC，and will generate( ) salient sticky end：（ ）
- A certain restricion endonuclease cut DNA by means of 5′…GGGGGG▼AATTCC…3,’ and will generate（ ）
- The plasmid used in DNA recombination techology is： （ ）
- Which of the following could not be used as vector for DNA cloning?（ ）
- Which of the following is the end that T4 ligase catalize to link together?（ ）
- General recombination is also called：（ ）
- Which of the following is the most general characteristic of the vector needed in DNA cloning?（ ）
- To construct cDNA library need：（ ）
- The process of DNA cloning does not include（ ）
A:yeast cell expression system B:insect expression system C:prokaryotic expression system D:E.coli expression system E:mammalia cell expression system
答案:mammalia cell expression system
A:5 nucleotides B:2 nucleotides C:4 nucleotides D:3 nucleotides E:1 nucleotide
A:blunt end B:5′salient(突出的) Sticky end C:5′and 3′salient sticky end D:3′salient sticky end E:5′or 3′salient sticky end
A:part of the eukaryotic chromosome genomic DNA B:Part of the cell chromosomal DNA C:the independent hereditary unit outside of eukaryotic chromosomal DNA D:part of the viral genomic DNA E:the independent hereditary unit outside of the becterial genomic DNA
A:reverse transcription DNA B:Bacterial genome DNA C:plasmid DNA D:adenovirus DNA E:phage DNA
A:5′-P and 3′-P B:any two end of the above C:5′-OH and 3′-P D:5′-P and 3′-OH E:5′-OH and 3′-OH
A:Site-specific Recombination B:natural recombination C:random recombination D:Homologous recombination E:man-made recombination
A:kanamycin resistance B:self replication ability C:Ampicillin resistance D:self transcription ability E:self exprssion ability
A:DNA polymerse and mRNA template B:DNA polymerase and tRNA template C:reverse transcriptase and mRNA template D:reverse transcriptase and rRNA template E:reverse transcriptase and DNA template
A:Screening for recombinant plasmids B:probe synthesis for target gene detection C:Transformation of target gene into receptor cell D:Selection and construction of vectors E:Isolation of target gene
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