- The crosslink between glycogenesis and glycogenolysis is （ ）
- Direct bilirubin is synthesized at : （ ）
- Oxidative phosphorylation occurs in: （ ）
- Substrate level phosphorylation is involved in （ ）
- Which of the following amino acid can not produce one carbon unit? （ ）
- What is gene expression?（ ）
- Which is an intermediate on the pathway for synthesis of ketone bodies and cholesterol?（ ）
- The content of which of the following element is close to each other in various protein?（ ）
- In which following conditions are ketone bodies synthesis greatly accelerated? （ ）
- he sub-cell site of cholesterol synthesis is （ ）
- Which of the following link the core particle of nucleosome ?（ ）
- Which of following is independent of Km?（ ）
- Which of the following metabolic reaction can not occur in liver ? （ ）
- The kinetics of enzymatic reaction is the study on：（ ）
- Which of the following is not concerned with the cholesterol biosynthesis?（ ）
- The effect of temperature on the rate of enzymatic reactions is （ ）
- Which of following is the major organ to regulate the concentration of glucose?（ ）
- Which of the following DNA has the lowest Tm? （ ）
- The translational initiation complex includes （ ）
- The major factor to control oxidative phosphorylation in normal physiological condition is：（ ）
- Which of the following is the product of the replication continuously: （ ）
- Which of the following hormone can accelerate fat mobilization?（ ）
- During the elongation of polypeptide chain in protein biosynthesis, which site can receipt the aminoacyl-tRNA（ ）
- Which of following is not the characteristics of enzymatic reactions? （ ）
- The α-subunit of G protein have the activity of：（ ）
- Malonate with succinate for succinate dehydrogenase is the classic case of：（ ）
- Which of following is not correct about competitive inhibitor? （ ）
- Which of the following is not correct about primary bile acid ? （ ）
- Which of the following is the characteristic of RNA-pol?（ ）
- The raw material of cholesterol in the body is （ ）
- Which of the following amino acid contain hydroxyl group?（ ）
- Every integrity protein must process: （ ）
- Where does de novo synthesis of purine nucleotide mainly take place?（ ）
- The kinetic property of non-competitive inhibition is：（ ）
- Which of the following is the enzyme of peptide bond formation ：（ ）
- Which of the following do not take part in the biotransformation of heme in liver ? （ ）
- Different enzymes catalyze sequential reactions in the same pathway are bound together in （ ）
- Energy for brain in long starvation mainly comes from （ ）
- After phosphorylation of glycogen synthase and phosphrylase （ ）
- The primary structure of DNA is the sequence of its nucletide residues linked by （ ）?
- Which of the following are related with DNA replication initiation?（ ）
- Which of following is correct about enzyme?（ ）
- Which of following is correct about covalent modification?（ ）
- Which of the following are the main transportation style of ammonia between tissues? （ ）
- Properties of binding of H and R are：（ ）
- The end product of gene expression could be：（ ）
- The DNA repairing type include:（ ）
- Which of the following about trans-acting factors are correct?（ ）
- Which of the following are the properties of α-helix structure?（ ）
- Which of the following could catalize the synthesis of phosphodiester bond?（ ）
- The plasmid used in DNA recombination techology is： （ ）
- A certain restricion endonuclease cut DNA by means of 5′…GGGGGG▼AATTCC…3,’ and will generate（ ）
- A certain restricion endonuclease cut DNA by means of GGG▼CGCCC，and will generate( ) salient sticky end：（ ）
- Which of the following could not be used as vector for DNA cloning?（ ）
- To construct cDNA library need：（ ）
- Which of the following is the most general characteristic of the vector needed in DNA cloning?（ ）
- The process of DNA cloning does not include（ ）
- Which of the following is the end that T4 ligase catalize to link together?（ ）
- General recombination is also called：（ ）
- The optimal cell system to express human protein is（ ）
- Which of the following about C-onc is correct?（ ）
- Which of the following about C-onc is not correct?（ ）
- Which of the following belong to anti-oncogene?（ ）
- Which of the following are not the expression product of oncogene?（ ）
- Which of the following element could activate oncogene?（ ）
- Which of the following does not belong to oncogene?（ ）
- Which of the following belong to oncogen expression product?（ ）
- Which of the following about oncogene is not correct?（ ）
- The oncogene would be activated in the following of situation：（ ）
- Which of the following could induce cell canceration?（ ）
- Which of the following belong to oncogene?（ ）
- Which of the following about properties of binding of hormone and receptor is not correct（ ）。
- The α subunit of G protein have the activity of（ ）。
- Which of the following does not belong to second messenger（ ）。
- Which of the following does not participate in AC-cAMP-PKA pathway（ ）。
- G protein is referred to（ ）。
- Which of the following do not participate in cellular signal transduction（ ）。
- Signal transduction pathway mediated by EGF receptor consist of （ ）。
- The chemical nature of intracellular receptors is（ ）。
- Which can cause endoplasmic reticulum release Ca2+（ ）。
- Extracellular chemical molecules include（ ）。
- Which of the following general transcription factors can recognize and bind to TATA box directly（ ）。
- Operons do not include（ ）。
- Which of the following belongs to a cis-acting element（ ）。
- Which of the following does not belong to gene expression（ ）。
- The structure of lac operon consist of（ ）。
- Which of the following belongs to a trans-acting factor （ ）。
- Which induces the transcription of lac operon（ ）。
- When the tryptophan levels are high, the tryptophan operon is regulated by（ ）。
- Which is bound by RNA polymerase and initiates transcription（ ）。
- The general regulatory point of gene expression is（ ）。
- The substance that is not needed for protein biosynthesis is（ ）。
- Which of the following tRNA anticodon is matched with mRNA codon ACG（ ）。
- Aminoacyl-tRNA synthetase has specificity for（ ）。
- Which enzyme catalyzes the formation of peptide bond （ ）。
- Which of the following material can carry the genetic information of DNA to the cytoplasm and to guide the biosynthesis of protein（ ）。
- What components are needed for the ribosome cycle（ ）。
- The introduction of eukaryotic secretory proteins into the endoplasmic reticulum is（ ）。
- The energy required for peptide chain elongation in prokaryotic protein biosynthesis is（ ）。
- In the characteristics of the codons, the frameshift mutation is related to（ ）。
- Substances involved in protein biosynthesis are（ ）。
- The transcript of RNA polymerase I in eukaryotes is（ ）
- Which subunit of RNA polymerase is the specific binding site of Rifampicin in prokaryotes?（ ）
- The transcript of template 5'-ACTAGTCAT -3' is （ ）
- The primary role of RNA polymerase Ⅲ is to synthesis （ ）
- Which one is correct about the exon? （ ）
- Which subunit of RNA polymerase can recognize the transcription start site in prokaryotes?（ ）
- The binding site of TFⅡD is （ ）
- Feather-like structure of transcription under electron microscope indicates（ ）
- Which one can inhibit prokaryotic RNA polymerase specifically? （ ）
- RNA holoenzyme includes σ subunit, it binds with DNA template strand at the initiation of the transcription. In the elongation stage，the σ subunit will （ ）
- Which of the following is the larger fragment of DNApolⅠ? （ ）
- The materials of DNA synthesis are：（ ）
- Which of these enzyme is not concerned with DNA replication？（ ）
- The prokaryotic DNA-pol core enzyme is made up with（ ）?
- Which of the following serve as the enzyme in mitochondria DNA replication ? （ ）
- In DNA replication, what is the reason of Okazaki fragment generated? （ ）
- DNA and RNA are composed of four types of deoxyribonucleotides and ribonucleotides ,respectively linked with （ ）?
- Which of the following is the product of the replication discontinuously?（ ）
- Which of the following could proofreading, DNA repairing, filling of gaps? （ ）
- In the initiation of DNA replication, the proper order that following enzyme and protein acting is? 1, DNA-polⅢ 2，SSB 3，primase 4, helicase（ ）
- In the prokaryotic DNA replication, which of the following is NOT needed? （ ）
- The common donors of the conjugated substances are （ ） in the phase II reaction of the liver.
- Which of the following is not the donator of biotransformation? （ ）
- Which is the most important enzyme taking part in the oxidation reaction of the biotransformation? （ ）
- The most important phase I reaction of the biotransformation is （ ）
- The most relevant of the biotransformation process in liver is （ ）
- The most significance of the biotransformation is to （ ）.
- The most important phase II reaction in the biotransformation is （ ）.
- Non-nutritional substance can （ ） after biotransformation.
- Which of the following can provide active methyl group ? （ ）
- Which of the following can provide active sulphate? （ ）
- Which of the following is the enzyme that catalyze the synthesis of uric acid ? （ ）
- Which of the following is not the direct material of de novo synthesis of purine nucleotide ? （ ）
- Which of the following substance directly link nucleotide synthesis and glucose metabolism? （ ）
- Which of the following is the methyl group donor of TMP ? （ ）
- Which of the following is the end production of purine nucleotide catabolic metabolism? （ ）
- Which of the following material could provide C4 and C5 for purine ring? （ ）
- Which of the following is the analogue of azaserine ? （ ）
- Which of the following is directly reduced, and produce deoxynucleotide? （ ）
- Which of the following is the common material for the synthesis of pyrimidine and purine nucleotide? （ ）
- Which of the following is first synthesized in the process of de novo synthesis of purine nucleotide? （ ）
- In the process of urea cycle, which of the following substance can go out mitochondrion? （ ）
- Which of the following is not produced by Tyr? （ ）
- Which of the following is not one carbon unit? （ ）
- In the synthesis of urea, one N come from NH3, another N come from （ ）directly ?
- Which of the following is not the outlet of α-keto acid that produced by deamination of amino acid? （ ）
- Which of the following is the carrier of one carbon unit ? （ ）
- Which of the following is the direct donor of CH3? （ ）
- Which of the following is the storage and transportation style of ammonia? （ ）
- Which of the following is only ketogenic amino acid? （ ）
- Which of the following amino acid can produce PAPS? （ ）
- The major function of HDL is （ ）
- Choline is activated in the form of （ ）, before it enters diacylglycerol pathway.
- The lipoprotein that transfer endogenous cholesterol is: （ ）
- Which of following does not utilize ketone bodies? （ ）
- Which is the major form that transports exogenous TAGs to the circulation? （ ）
- Which is the major form that transports endogenous TAGs from the liver to the tissues? （ ）
- Which is not required in β-Oxidation of fatty acids? （ ）
- In the liver, （ ） is the rate–limiting enzyme on the pathway for synthesis of cholesterol.
- How many mols of ATP are yielded when 1 mol stearic acid (18 C) is oxidized to CO2 and H2O ? （ ）
- NADPH in fatty acid synthesis comes from: （ ）
- The characteristic of biological oxidation is（ ）
- Which of the following enzyme uses FAD as prothetic group?（ ）
- The coenzyme of α-glycerophosphate dehydrogenase in mitochondria is:（ ）
- Which of the following metabolic process is completed in mitochondrion totally （ ）
- Which of following is the carrier of NADH from cytosol to mitchondria?（ ）
- Which of the following is not the energy-rich compound? （ ）
- Which agent is an inhibitor of electron transport at Complex Ⅲ?（ ）
- How many ATPs will be produced when 1 cytosolic NADH is transported into the mitochondria by α-glycerophosphate shuttle?（ ）
- The order of NADH respiratory chain is:（ ）
- Which of the following is the most important element in the regulation of oxidative phosphorylation?（ ）
- Which is metabolized through both the glycolytic pathway and the pentose phosphate pathway?（ ）
- How many ATPs are produced by the oxidation of a molecule of aceyl CoA via the citric acid cycle?
- Substrate-level phosphorylation in citrate acid cycle is:（ ）
- The end-product of glycolysis is: （ ）
- The key enzyme of the glycolysis is: （ ）
- Why erythrocytes metabolize glucose by anaerobic glycolysis? （ ）
- Which of following is not the key enzyme in aerobic oxidation of carbohydrate?（ ）
- 1 molecule of pyruvate which is oxidized completely may generate ( ) molecules of ATP. （ ）
- Which of following generate FADH2 after dehydrogenation? （ ）
- What are the most important products the cells generate by means of the pentose phosphate pathway?（ ）
- Which of following is not correct about LDH isoenzymes?（ ）
- The relationship between competitive inhibitor and substrate is that they have :（ ）
- Which of following is not correct about Km?（ ）
- Which of following is correct about character of enzyme?（ ）
- Which of following is not correct about allosteric enzyme? （ ）
- Isoenzymes are enzymes that ：（ ）
- Which of following is not correct about active site of an enzyme?（ ）
- Which of following is not correct about covalent modification?（ ）
- Specificity of enzyme refers to：（ ）
- 5’-end of the majority of mRNA in eukaryotes has （ ）
- DNA can be separated into two single chains during heating （ ）
- The template of protein synthesis is （ ）
- Which statement is true for Tm? （ ）
- 3'-end of mRNA has （ ）
- The RNA that can carry amino acid and transfer it to ribosome is （ ）
- The tertiary structure of tRNA is （ ）
- The secondary structure of DNA is （ ）
- Which statement is NOT true for RNA? （ ）
- Which statement is NOT true for tRNA? （ ）
- All the common proein absorb light at a wavelength of UV close to （ ） nm?
- One AA( pI=5.6 ) will be negatively charged in a solution （ ）?
- Which of the followings is basic AA? （ ）
- α helix of protein structure belongs to（ ）
- The amino acid found as constituents of mammalian proteins are all ( ) except for glycine?（ ）
- The nitrogen content of one protein sample is 0.5g，and then the protein content of it is ：（ ）
- Which of the following about protein secondary structure is not correct? （ ）
- Which of the following about quaternary structue is correct? （ ）
- Which of the following about protein denaturaion is not correct?（ ）
- Which of the following about α-helix is not correct：（ ）
- Biochemistry can be divided into three principal areas, that is：（ ）
答案:glucose 1-phosphate
答案:liver
答案:mitochondria
答案:1,3-bisphosphoglycerate→3-phosphoglycerate
答案:Valine
答案:transcription , translation
答案:HMG-CoA
答案:N
答案:fat mobilization is enhanced
答案:cytosol+ endoplasmic reticulum
答案:H1
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