第十章单元测试
- Which of the following statements is not true concerning Type II restriction endonucleases?（ ）
- Which is not necessary as part of prokaryotic expression vector?（ ）
- Based on the naming rules, the first letter of a restriction endonuclease means（ ）
- Isocaudarner presents（ ）
- Plasmids are usually introduced into bacterial cells by.（ ）
- T4 DNA ligase requires（ ）
- In insertional inactivation screening, the bacteria that are sensitive to tetracycline and resistant to ampicillin should be transformed with（ ）
- The loading capacity of plasmids is usually （ ）
- Which of the following methods is used for isolation of foreign DNA（ ）
- Which of the following DNA molecules are commonly used in molecular cloning（ ）
- Restriction enzymes randomly cleave DNA（ ）
- In blue/white screening, the bacteria containing wild vectors will yield white colonies.（ ）
A:They can cleave single DNA B:Cut DNA into fragments at recognition site C:Recognize palindrome sequence D:Sticky ends or blunt ends are produced after digestion
答案:They can cleave single DNA
A:Poly A signal B:Promoter C:Ribosome binding site D:Selectable markers
A:Identified order B:Bacterial strain C:Bacterial species D:Bacterial genus
A:Various restriction endonucleases recognize same palindromes, and produce same sticky ends B:Various restriction endonucleases recognize different palindromes, but produce same sticky ends C:Various restriction endonucleases recognize same palindrome, but cut at identical site D:Various restriction endonucleases recognize same palindrome, but cut at different site
A:Infection B:Transfection C:Transformation D:Transduction
A:ATP B:NAD+ C:GTP D:AMP
A:Recombinant DNA B:Foreign DNA only C:Self-cyclized vectors D:Nothing
A:Less than 10kb B:Up to 45kb C:More than 20kb D:More than10kb
A:DNA library B:Chemical synthesis C:Yeast two-hybrid system D:PCR
A:BAC B:Cosmid C:Bacteriophage D:Plasmid
A:错 B:对
A:对 B:错
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