知到答案生物化学（英文版）智慧树答案_2022年

第十四章

Pipettes should be used only with Rubber Bulbs, mouth pipetting should not be allowed.
答案:对
The protein has the properties of UV absorption, which has the maximum absorption peak at 260nm.The ration of A260 to A280 can be used to evaluate the purity of extracted DNA.
答案:对
Cuvette can't be cleaned with a strong base, but can be immersed in lotion or 1%-2% decontaminant, then washed with tap water and distilled water.
答案:对
A spectrophotometer is employed to measure the amount of light that a sample absorbs.
答案:对
Which of the following technologies is not protein chromatography （ ）
ion exchange
gel filtration
salting out
hydrophobic chromatographyWhich of the following is not a common method for protein content determination （ ）
ultraviolet absorption method
BCA method
biuret reagent method
Folin reagent methodWhich of the following reagent cannot be used as a component of the sample loading buffer in SDS-PAGE （ ）
mercaptoethanol
glycerine
bromophenol blue
glacial acetic acidThe principle of ammonium sulfate precipitation of protein is （ ）
denaturation of proteins
to neutralize the surface charge and destroy the hydration membrane of the protein
the density of protein is greater than that of water
the charge of protein is opposite to that of ammonium sulfateIn PCR reaction, the annealing temperature is generally set as （ ）
55-65℃
100℃
25-30℃
72℃