- Glycolysis takes place in mitochondria.（ ）
- Ion-exchange chromatography is a method that can be used to separate and purify proteins, basing on the relative molecular mass of proteins.（ ）
- All enzymes involved in gluconeogenesis exist in the cytoplasm.（ ）
- RNAs are synthesized from DNA templates, no need for a primer.（ ）
- β-Oxidation of FAs includes five repeated steps: activation, oxidation, hydration, oxidation and thiolysis. （ ）
- The base thickness of α-helix is 3.4 nm.（ ）
- Tyrosine is (are) neutral and polar at pH = 7.（ ）
- A number of the digestive enzymes are synthesized in an inactive form and are only activated in the duodenum. These inactive precursor forms are termed（ ）.
- Urea is utilized in（ ）.
- Which of the following does not pump protons? （ ）.
- All of the following compounds contain high-energy phosphate bond, EXCEPT（ ）.
- What are the steps involved (in order) in the conversion of pyruvate to acetyl CoA?（ ）
- For identification of a gene, against which strand of DNA must the probe complement: the top or bottom strand? （ ）.
- The chemical bond in charge of maintaining secondary structure of proteins is（ ）.
- All of the following amino acids are L-amino acids, EXCEPT（ ）.
- All of the following processes occur in the mitochondria, EXCEPT（ ）.
- The reduced form of flavin adenine dinucleotide is（ ）.
- Name three amino acids that are positively charged at a neutral pH（ ）.
- The wavelength of maximum absorption of nucleic acids is 280 nm.（ ）
- Ketone bodies are produced in the mitochondrial matrix of liver cells, and then further oxidized in liver cells, which is an another form of oxidative energy supply for fatty acids.（ ）
- The kinetic behavior of allosteric enzymes does not follow the Mechaelis-Menten equation.（ ）
- Three nucleotides code for 3 amino acids.（ ）
- Genes can be inserted into eukaryotic cells by （ ）.
- Which of the following amino acid residues would most likely be buried in the interior of a water soluble, globular protein（ ）?
- What are the primary metabolic fates of pyruvate?（ ）
- The enzyme in charge of proofreading function in DNA replication is（ ）.
- The sites for intron splicing consensus sequences are often（ ）.
- Cori cycle is the process that transport ammonia from muscle to liver?（ ）
- When a protein is denatured, its molecular mass decreases.（ ）
- In the process of DNA replication, the enzyme that can remove primers is DNA polymeraseⅠ.（ ）
- Most eukaryotic mRNAs have poly(A) at the 5 'end and cap structure at the 3' end.（ ）
- The coenzyme of most aminotransferases is pyridoxal phosphate.（ ）
- All enzymes involved in glycolysis exist in the cytoplasm.（ ）
- Alanine-glucose cycle is the process that transport ammonia from muscle to liver?（ ）
- ATP is the main form of energy storage.（ ）
- The initial synthesis of polypeptide chains of prokaryotes begin with formylmethionine (fMet).（ ）
- The wavelength of maximum absorption of proteins is 260 nm.（ ）
- Reverse transcriptase is normally found in（ ）.
- Ribosomes are composed of（ ）.
- Which of the following is the main deamination style in the body?（ ）
- If a plot of the reciprocal of the velocity of an enzyme catalyzed reaction versus the reciprocal of the substrate concentration yields a straight line, the intercept on the 1/[S] axis is（ ）.
- The site to which RNA polymerase binds on the DNA template prior to the initiation of transcription is （ ）.
- The difference in RNA bases compared to DNA bases is（ ）.
- What do Southern, Northern, and Western blots detect, respectively?（ ）
- In the urea cycle, the carbon skeleton of aspartate is preserved as（ ）.
- What is the binding site of peptidyl-tRNA?（ ）
- The first amino acid in bacterial proteins is（ ）.
- How many high-energy phosphate bonds are expended in gluconeogenesis?（ ）
- Which of the following is an allosteric activator of mammalian carbamoyl-phosphate synthetase?（ ）
- In protein synthesis, after the initiation complex forms, how many high-energy phosphate bonds will be consumed when an amino acid is added into a peptide chain?（ ）
- The Km is（ ）.
- What is the approximate error rate in DNA replication?（ ）
- If the codon of mRNA is ACG, then the anticodon of tRNA is（ ）.
- Which of the following sequences would retain the base-pairing necessary to form a hairpin loop? （ ）The original sequence is UUGCUCAGUAAGAGCAA.
- Plasmids used in recombinant DNA technology typically（ ）.
- Which of the following codon are the initiation codon in the process of protein biosynthesis? （ ）
- How many rounds of oxidation would be required for a 16-C fatty acyl chain to be degraded to acetyl-CoA?（ ）
- Which of the following steps is NOT included in the protein biosynthesis?（ ）
- The energy required for protein synthesis is ATP（ ）.
- Promoter is a short sequence rich in purines, lies 5’to the AUG initiation codon and is complementary to part of the 16S rRNA in the small ribosomal subunit（ ）.
- Energy for the movement of peptidyl-tRNA from the A-site to the P-site is provided by（ ）.
- The operon model proposes three elements, EXCEPT（ ）.
- Most mRNAs of eukaryotes are polycistron, while most mRNAs of prokaryotes are monocistron（ ）.
- The RNA polymerase holoenzyme of prokaryotes is composed of several subunits, such as α2ββω（ ）.
- The template strand of DNA is 5’-ATTCAG-3’, its transcript is（ ）.
- Which of the following codons are the termination codons in protein biosynthesis?（ ）
- All of the following descriptions about the synthesis of leading strands in DNA replication in prokaryoticcells are correct, EXCEPT（ ）.
- The leading strand is continuously replicated, and the lagging strand is not continuously replicated, which is the semi-conservative replication（ ）.
- During deamination, L-amino acid oxidase has higher activity and is found widely in bodies（ ）.
- Urea is synthesized in（ ）.
- In the urea cycle, one “N” in the urea molecule comes from NH3, another “N”comes from（ ）
- Under All of the following molecules are called ketone bodies, EXCEPT（ ）.
- The substances that can produce acetyl CoA are （ ）.
- Unbranched fatty acids with an odd number of carbon atoms are usually converted after β-oxidation to acetoacetyl-CoA and acetyl-CoA（ ）.
- For each molecule of glucose converted to pyruvate in the glycolytic pathway ___molecules of ATP are used initially (Stage I) and ___ molecules of ATP are produced (Stage II) for an overall yield of ___ molecules of ATP/ glucose. The "ATP math" is:（ ）.
- Which of the following reactions is not a control point in the citric acid cycle?（ ）.
- Complex II of the electron transport chain shares its enzymatic activity with the citric acid cycle.（ ）
- Which compound is NOT the coenzyme of pyruvate dehydrogenase complex?（ ）
- The sequence of cytochrome in the respiratory chain is b→c1→c→aa3→O2.（ ）
- The order of the members of NADH oxidative respiratory chain is（ ）.
- All of the following compounds are components of the respiratory chain, EXCEPT（ ）.
- Two hydrogen atoms (2H) in cytosolic NADH(+H+) are transported from cytosol to mitochondria through（ ）.
- About competitive inhibitors, which one is true?（ ）.
- Isoenzyme is a group of enzymes that catalyze the same chemical reaction, and the molecular structure, physicochemical properties and immunological properties of the enzyme protein are different（ ）.
- Inhibition of phosphate pesticide on choline esterase belongs to（ ）.
- Northern hybridization is used to detect（ ）.
- The correct description about measuring DNA denaturation are included, EXCEPT（ ）.
- The sedimentation coefficient of the mammalian ribosome large subunit is 50S.（ ）
- The protein molecule (PI=4.5) will mobile to the negative electrode when the PH of electrophoresis buffer is（ ）.
- Gel filtration chromatography is a method that can be used to separate and purify proteins, basing on the solubility of proteins（ ）
- All of the following amino acids are acidic or basic amino acids, EXCEPT（ ）.
- The amino acids that determine the UV-absorbing properties of proteins are（ ）.
- Approximately what percentage of the human genome encodes for proteins?（ ）
答案:错
答案:错
答案:错
答案:对
答案:错
答案:错
答案:对
答案:Zymogens
答案:Extra-hepatic
答案:Complex II
答案:Glucose-6-phosphate
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